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IMAC capture of recombinant protein from unclarified mammalian cell feed streams
Fusion‐tag affinity chromatography is a key technique in recombinant protein purification. Current methods for protein recovery from mammalian cells are hampered by the need for feed stream clarification. We have developed a method for direct capture using immobilized metal affinity chromatography (...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Biotechnol Bioeng |
|---|---|
| Prif Awduron: | , , , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
John Wiley and Sons Inc.
2015
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4737217/ https://ncbi.nlm.nih.gov/pubmed/26174988 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.25705 |
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