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Oxidation of Phe454 in the Gating Segment Inactivates Trametes multicolor Pyranose Oxidase during Substrate Turnover

The flavin-dependent enzyme pyranose oxidase catalyses the oxidation of several pyranose sugars at position C-2. In a second reaction step, oxygen is reduced to hydrogen peroxide. POx is of interest for biocatalytic carbohydrate oxidations, yet it was found that the enzyme is rapidly inactivated und...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Halada, Petr, Brugger, Dagmar, Volc, Jindrich, Peterbauer, Clemens K., Leitner, Christian, Haltrich, Dietmar
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4735113/
https://ncbi.nlm.nih.gov/pubmed/26828796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0148108
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