Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching

We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca(2+) events in primary rat hippoc...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Amor, Rumelo, McDonald, Alison, Trägårdh, Johanna, Robb, Gillian, Wilson, Louise, Abdul Rahman, Nor Zaihana, Dempster, John, Amos, William Bradshaw, Bushell, Trevor J., McConnell, Gail
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4732674/
https://ncbi.nlm.nih.gov/pubmed/26824845
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0147115
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