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High-Throughput Isolation of Giant Viruses in Liquid Medium Using Automated Flow Cytometry and Fluorescence Staining

The isolation of giant viruses using amoeba co-culture is tedious and fastidious. Recently, the procedure was successfully associated with a method that detects amoebal lysis on agar plates. However, the procedure remains time-consuming and is limited to protozoa growing on agar. We present here adv...

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Veröffentlicht in:Front Microbiol
Hauptverfasser: Khalil, Jacques Y. B., Robert, Stephane, Reteno, Dorine G., Andreani, Julien, Raoult, Didier, La Scola, Bernard
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4731542/
https://ncbi.nlm.nih.gov/pubmed/26858703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.00026
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