Production of unmodified human adult hemoglobin in Escherichia coli.

We have constructed a plasmid (pHE2) in which the synthetic human alpha- and beta-globin genes and the methionine aminopeptidase (Met-AP) gene from Escherichia coli are coexpressed under the control of separate tac promoters. The Hbs were expressed in E. coli JM109 and purified by fast protein liqui...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Shen, T J, Ho, N T, Simplaceanu, V, Zou, M, Green, B N, Tam, M F, Ho, C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47297/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367471/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.17.8108
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