Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli

BACKGROUND: Disulfide bonds are the most common structural, post-translational modification found in proteins. Antibodies contain up to 25 disulfide bonds depending on type, with scFv fragments containing two disulfides and Fab fragments containing five or six disulfide bonds. The production of anti...

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Bibliografiska uppgifter
I publikationen:Microb Cell Fact
Huvudupphovsmän: Gaciarz, Anna, Veijola, Johanna, Uchida, Yuko, Saaranen, Mirva J., Wang, Chunguang, Hörkkö, Sohvi, Ruddock, Lloyd W.
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2016
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4727284/
https://ncbi.nlm.nih.gov/pubmed/26809624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0419-5
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