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Peptides in headlock – a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy

Nanobodies are highly valuable tools for numerous bioanalytical and biotechnical applications. Here, we report the characterization of a nanobody that binds a short peptide epitope with extraordinary affinity. Structural analysis reveals an unusual binding mode where the extended peptide becomes par...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Braun, Michael B., Traenkle, Bjoern, Koch, Philipp A., Emele, Felix, Weiss, Frederik, Poetz, Oliver, Stehle, Thilo, Rothbauer, Ulrich
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4726124/
https://ncbi.nlm.nih.gov/pubmed/26791954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep19211
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