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An efficient full-length cDNA amplification strategy based on bioinformatics technology and multiplexed PCR methods

A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. The amplification of 3′ ends of cDNA is performed according to the modified classic 3′ RACE techniques, therein the more efficient...

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Detaylı Bibliyografya
Yayımlandı:Sci Rep
Asıl Yazarlar: Chen, Nan, Wang, Wei-Min, Wang, Huan-Ling
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group 2016
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4725349/
https://ncbi.nlm.nih.gov/pubmed/26758040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep19420
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