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Primary hepatocyte culture in collagen gel mixture and collagen sandwich

AIM: To explore the methods of hepatocytes culture in a collagen gel mixture or between double layers of collagen sandwich configuration and to examine the functional and cytomorphological characteristics of cultured hepatocytes. METHODS: A two-step collagenase perfusion technique was used to isolat...

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Publicado en:World J Gastroenterol
Autores principales: Wang, Ying-Jie, Liu, Hong-Ling, Guo, Hai-Tao, Wen, Hong-Wei, Liu, Jun
Formato: Artigo
Lenguaje:Inglês
Publicado: Baishideng Publishing Group Inc 2004
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4716912/
https://ncbi.nlm.nih.gov/pubmed/14991941
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i5.699
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