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A novel assay to identify the trafficking proteins that bind to specific vesicle populations
Here we describe a method capable of identifying interactions between candidate trafficking proteins and a defined vesicle population in intact cells. The assay involves the expression of an FKBP12-rapamycin–binding domain (FRB)–tagged candidate vesicle-binding protein that can be inducibly linked t...
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| Udgivet i: | Curr Protoc Cell Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4714922/ https://ncbi.nlm.nih.gov/pubmed/26621371 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471143030.cb1308s69 |
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