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Novel reporter system to monitor early stages of the hepatitis B virus life cycle

A recombinant hepatitis B virus (HBV) expressing NanoLuc (NL) (HBV/NL) was produced by cotransfecting a plasmid containing a 1.2‐fold HBV genome carrying the NL gene with a plasmid bearing a packaging‐defective 1.2‐fold HBV genome into a human hepatoma cell line, HepG2. We found that NL activity in...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Cancer Sci
Hauptverfasser: Nishitsuji, Hironori, Ujino, Saneyuki, Shimizu, Yuko, Harada, Keisuke, Zhang, Jing, Sugiyama, Masaya, Mizokami, Masashi, Shimotohno, Kunitada
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4714683/
https://ncbi.nlm.nih.gov/pubmed/26310603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/cas.12799
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