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Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts

Mammalian cells encode a diverse set of Rab GTPases and their corresponding regulators. In vitro biochemical screening has proven invaluable in assigning particular Rabs as substrates for their cognate GTPase-activating proteins. However, in vitro activity does not always reflect substrate specifici...

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Bibliografski detalji
Izdano u:Methods Mol Biol
Glavni autori: Nottingham, Ryan M., Pfeffer, Suzanne R.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4710138/
https://ncbi.nlm.nih.gov/pubmed/25800832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2569-8_5
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