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A Rapid and Cheap Methodology for CRISPR/Cas9 Zebrafish Mutant Screening

The introduction of new genome editing tools such as ZFNs, TALENs and, more recently, the CRISPR/Cas9 system, has greatly expanded the ability to knock-out genes in different animal models, including zebrafish. However, time and costs required for the screening of a huge number of animals, aimed to...

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Bibliographic Details
Published in:Mol Biotechnol
Main Authors: D’Agostino, Ylenia, Locascio, Annamaria, Ristoratore, Filomena, Sordino, Paolo, Spagnuolo, Antonietta, Borra, Marco, D’Aniello, Salvatore
Format: Artigo
Language:Inglês
Published: Springer US 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4709366/
https://ncbi.nlm.nih.gov/pubmed/26676479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12033-015-9905-y
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