Benefits and Challenges with Applying Unique Molecular Identifiers in Next Generation Sequencing to Detect Low Frequency Mutations

Indexing individual template molecules with a unique identifier (UID) before PCR and deep sequencing is promising for detecting low frequency mutations, as true mutations could be distinguished from PCR errors or sequencing errors based on consensus among reads sharing same index. In an effort to de...

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Udgivet i:PLoS One
Main Authors: Kou, Ruqin, Lam, Ham, Duan, Hairong, Ye, Li, Jongkam, Narisra, Chen, Weizhi, Zhang, Shifang, Li, Shihong
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2016
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4709065/
https://ncbi.nlm.nih.gov/pubmed/26752634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0146638
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