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Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst

Enzymes expressed by highly salt-tolerant organisms show many modifications compared with salt-affected counterparts including biased amino acid and lower α-helix content, lower solvent accessibility and negative surface charge. Here, we show that halotolerance can be generated in an enzyme solely b...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Warden, Andrew C., Williams, Michelle, Peat, Thomas S., Seabrook, Shane A., Newman, Janet, Dojchinov, Greg, Haritos, Victoria S.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4703901/
https://ncbi.nlm.nih.gov/pubmed/26687908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10278
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