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Overexpression, characterization, and purification of a recombinant mouse immunophilin FKBP-52 and identification of an associated phosphoprotein.

To gain insight into the structure and function of the immunophilin FKBP-52, a mouse FKBP-52 was overexpressed in Spodoptera frugiperda insect cells (Sf9 cells) with the baculovirus expression system. The purification and characterization of the recombinant FKBP-52 (rFKBP-52) was facilitated by inco...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Alnemri, E S, Fernandes-Alnemri, T, Nelki, D S, Dudley, K, DuBois, G C, Litwack, G
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC47028/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8341706/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.14.6839
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