Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease...

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Detalhes bibliográficos
Publicado no:J Mol Diagn
Main Authors: Ramírez, Juan Carlos, Cura, Carolina Inés, Moreira, Otacilio da Cruz, Lages-Silva, Eliane, Juiz, Natalia, Velázquez, Elsa, Ramírez, Juan David, Alberti, Anahí, Pavia, Paula, Flores-Chávez, María Delmans, Muñoz-Calderón, Arturo, Pérez-Morales, Deyanira, Santalla, José, Guedes, Paulo Marcos da Matta, Peneau, Julie, Marcet, Paula, Padilla, Carlos, Cruz-Robles, David, Valencia, Edward, Crisante, Gladys Elena, Greif, Gonzalo, Zulantay, Inés, Costales, Jaime Alfredo, Alvarez-Martínez, Miriam, Martínez, Norma Edith, Villarroel, Rodrigo, Villarroel, Sandro, Sánchez, Zunilda, Bisio, Margarita, Parrado, Rudy, Galvão, Lúcia Maria da Cunha, da Câmara, Antonia Cláudia Jácome, Espinoza, Bertha, de Noya, Belkisyole Alarcón, Puerta, Concepción, Riarte, Adelina, Diosque, Patricio, Sosa-Estani, Sergio, Guhl, Felipe, Ribeiro, Isabela, Aznar, Christine, Britto, Constança, Yadón, Zaida Estela, Schijman, Alejandro G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4698797/
https://ncbi.nlm.nih.gov/pubmed/26320872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2015.04.010
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