A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpress(TM)

We developed an efficient, automated 2-step purification protocol for the production of milligram quantities of untagged recombinant rat lactate dehydrogenase A (rLDHA) from E. coli, using the ÄKTAxpress™ chromatography system. Cation exchange followed by size exclusion results in average final puri...

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Bibliografske podrobnosti
izdano v:PLoS One
Main Authors: Nowicki, Matthew W., Blackburn, Elizabeth A., McNae, Iain W., Wear, Martin A.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2015
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4696747/
https://ncbi.nlm.nih.gov/pubmed/26717415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0146164
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