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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Indirect immunofluorescence is used to detect and locate proteins of interest in a tissue. The protocol presented here describes a complete and simple method for the immune detection of proteins, the mouse lactating mammary gland being taken as an example. A protocol for the preparation of the tissu...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Honvo-Houéto, Edith, Truchet, Sandrine
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692769/
https://ncbi.nlm.nih.gov/pubmed/26650781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53179
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