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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
Indirect immunofluorescence is used to detect and locate proteins of interest in a tissue. The protocol presented here describes a complete and simple method for the immune detection of proteins, the mouse lactating mammary gland being taken as an example. A protocol for the preparation of the tissu...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4692769/ https://ncbi.nlm.nih.gov/pubmed/26650781 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53179 |
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