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Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Specific metabolic pathways are increasingly being recognized as critical hallmarks of macrophage subsets. While LPS-induced classically activated M1 or M((LPS) )macrophages are pro-inflammatory, IL-4 induces alternative macrophage activation and these so-called M2 or M((IL-4)) support resolution of...

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Pubblicato in:J Vis Exp
Autori principali: Van den Bossche, Jan, Baardman, Jeroen, de Winther, Menno P.J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2015
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692751/
https://ncbi.nlm.nih.gov/pubmed/26649578
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53424
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