Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

MscL, a large conductance mechanosensitive channel (MSC), is a ubiquitous osmolyte release valve that helps bacteria survive abrupt hypo-osmotic shocks. It has been discovered and rigorously studied using the patch-clamp technique for almost three decades. Its basic role of translating tension appli...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Najem, Joseph S., Dunlap, Myles D., Yasmann, Anthony, Freeman, Eric C., Grant, John W., Sukharev, Sergei, Leo, Donald J.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2015
Teme:
Bioengineering
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692740/
https://ncbi.nlm.nih.gov/pubmed/26650467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53362
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