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Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

Dysfunctional skeletal muscle mitochondria play a role in altered metabolism observed with aging, obesity and Type II diabetes. Mitochondrial respirometric assays from isolated mitochondrial preparations allow for the assessment of mitochondrial function, as well as determination of the mechanism(s)...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Boutagy, Nabil E., Pyne, Emily, Rogers, George W., Ali, Mostafa, Hulver, Matthew W., Frisard, Madlyn I.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692713/
https://ncbi.nlm.nih.gov/pubmed/26650566
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53217
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