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Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

We introduce a new method to measure the lateral diffusivity of a surfactant monolayer at the fluid-fluid interface, called fluorescence recovery after merging (FRAM). FRAM adopts the same principles as the fluorescence recovery after photobleaching (FRAP) technique, especially for measuring fluores...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Jeong, Dae-Woong, Kim, KyuHan, Choi, Myung Chul, Choi, Siyoung Q.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692663/
https://ncbi.nlm.nih.gov/pubmed/26556128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53376
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