Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

3,4-Methylenedioxymethamphetamine (MDMA; ecstasy) toxicity may cause region-specific changes in serotonergic mRNA expression due to acute serotonin (5-hydroxytryptamine; 5-HT) syndrome. This hypothesis can be tested using in situ hybridization to detect the serotonin 5-HT(2A) receptor gene htr2a. In...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Shokry, Ibrahim M., Callanan, John J., Sousa, John, Tao, Rui
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2015
Subjects:
Neuroscience
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692616/
https://ncbi.nlm.nih.gov/pubmed/26437182
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53165
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