Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Absolute quantification of target proteins within complex biological samples is critical to a wide range of research and clinical applications. This protocol provides step-by-step instructions for the development and application of quantitative assays using selected reaction monitoring (SRM) mass sp...

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Vydáno v:J Vis Exp
Hlavní autoři: Manes, Nathan P., Mann, Jessica M., Nita-Lazar, Aleksandra
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2015
Témata:
Biochemistry
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692538/
https://ncbi.nlm.nih.gov/pubmed/26325288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52959
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