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Adaptive optics in multiphoton microscopy: comparison of two, three and four photon fluorescence

We demonstrate adaptive optics system based on nonlinear feedback from 3- and 4-photon fluorescence. The system is based on femtosecond pulses created by soliton self-frequency shift of a 1550-nm fiber-based femtosecond laser together with micro-electro-mechanical system (MEMS) phase spatial light m...

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Publicado en:Opt Express
Main Authors: Sinefeld, David, Paudel, Hari P., Ouzounov, Dimitre G., Bifano, Thomas G., Xu, Chris
Formato: Artigo
Idioma:Inglês
Publicado: Optical Society of America 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4692258/
https://ncbi.nlm.nih.gov/pubmed/26698772
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.23.031472
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