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Thinned-skull cranial window technique for long-term imaging of the cortex in live mice

Imaging neurons, glia and vasculature in the living brain has become an important experimental tool for understanding how the brain works. Here we describe in detail a protocol for imaging cortical structures at high optical resolution through a thinned-skull cranial window in live mice using two-ph...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Yang, Guang, Pan, Feng, Parkhurst, Christopher N, Grutzendler, Jaime, Gan, Wen-Biao
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4690457/
https://ncbi.nlm.nih.gov/pubmed/20134419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.222
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