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Simple and inexpensive ribosome profiling analysis of mRNA translation

The development and application of ribosome profiling has markedly advanced our understanding of ribosomes and mRNA translation. The experimental approach, which relies on deep sequencing of ribosome-protected mRNA fragments generated by treatment of polyribosomes with exogenous nucleases, provides...

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Publicat a:Methods
Autors principals: Reid, David W., Shenolikar, Shirish, Nicchitta, Christopher V.
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4684803/
https://ncbi.nlm.nih.gov/pubmed/26164698
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2015.07.003
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