Development of Fok-I based nested polymerase chain reaction-restriction fragment length polymorphism analysis for detection of hepatitis B virus X region V5M mutation

AIM: To develop a Fok-I nested polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) method for the detection of hepatitis B virus X region (HBx) V5M mutation. METHODS: Nested PCR was applied into DNAs from 198 chronic patients at 2 different stages [121 patients wi...

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Enregistré dans:
Détails bibliographiques
Publié dans:World J Gastroenterol
Auteurs principaux: Kim, Hong, Hong, Seok-Hyun, Lee, Seoung-Ae, Gong, Jeong-Ryeol, Kim, Bum-Joon
Format: Artigo
Langue:Inglês
Publié: Baishideng Publishing Group Inc 2015
Sujets:
Observational Study
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4679770/
https://ncbi.nlm.nih.gov/pubmed/26715821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v21.i47.13360
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