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Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells

Many types of studies require the localization of a protein to, or isolation of enriched protein from a specific cellular compartment. Many protocols in the literature and from commercially available kits claim to yield pure cellular fractions. However, in our hands, the former often do not work eff...

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Bibliografische gegevens
Gepubliceerd in:MethodsX
Hoofdauteurs: Baghirova, Sabina, Hughes, Bryan G., Hendzel, Michael J., Schulz, Richard
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2015
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4678309/
https://ncbi.nlm.nih.gov/pubmed/26740924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2015.11.001
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