Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide.
Random mutagenesis has been used to engineer the protease subtilisin E to function in a highly nonnatural environment--high concentrations of a polar organic solvent. Sequential rounds of mutagenesis and screening have yielded a variant (PC3) that hydrolyzes a peptide substrate 256 times more effici...
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| Опубліковано в:: | Proc Natl Acad Sci U S A |
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| Автори: | , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
National Academy of Sciences
1993
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC46772/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8516309/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.12.5618 |
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