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Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination-Mediated Cassette Exchange

Transfection of transgenes into Drosophila cultured cells is a standard approach for studying gene function. However, the number of transgenes present in the cell following transient transfection or stable random integration varies, and the resulting differences in expression level affect interpreta...

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Dades bibliogràfiques
Publicat a:Genetics
Autors principals: Manivannan, Sathiya N., Jacobsen, Thomas L., Lyon, Peter, Selvaraj, Bhavani, Halpin, Peter, Simcox, Amanda
Format: Artigo
Idioma:Inglês
Publicat: Genetics Society of America 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4676529/
https://ncbi.nlm.nih.gov/pubmed/26500255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.181230
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