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Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination-Mediated Cassette Exchange

Transfection of transgenes into Drosophila cultured cells is a standard approach for studying gene function. However, the number of transgenes present in the cell following transient transfection or stable random integration varies, and the resulting differences in expression level affect interpreta...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Genetics
Prif Awduron: Manivannan, Sathiya N., Jacobsen, Thomas L., Lyon, Peter, Selvaraj, Bhavani, Halpin, Peter, Simcox, Amanda
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Genetics Society of America 2015
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4676529/
https://ncbi.nlm.nih.gov/pubmed/26500255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.181230
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