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Targeted Integration of Single-Copy Transgenes in Drosophila melanogaster Tissue-Culture Cells Using Recombination-Mediated Cassette Exchange

Transfection of transgenes into Drosophila cultured cells is a standard approach for studying gene function. However, the number of transgenes present in the cell following transient transfection or stable random integration varies, and the resulting differences in expression level affect interpreta...

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Detalles Bibliográficos
Publicado en:Genetics
Main Authors: Manivannan, Sathiya N., Jacobsen, Thomas L., Lyon, Peter, Selvaraj, Bhavani, Halpin, Peter, Simcox, Amanda
Formato: Artigo
Idioma:Inglês
Publicado: Genetics Society of America 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4676529/
https://ncbi.nlm.nih.gov/pubmed/26500255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.115.181230
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