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Automated Analysis of Single-Molecule Photobleaching Data by Statistical Modeling of Spot Populations

The number of fluorophores within a molecule complex can be revealed by single-molecule photobleaching imaging. A widely applied strategy to analyze intensity traces over time is the quantification of photobleaching step counts. However, several factors can limit and bias the detection of photobleac...

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Detalles Bibliográficos
Publicado en:Biophys J
Main Authors: Liesche, Clarissa, Grußmayer, Kristin S., Ludwig, Michael, Wörz, Stefan, Rohr, Karl, Herten, Dirk-Peter, Beaudouin, Joël, Eils, Roland
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4675885/
https://ncbi.nlm.nih.gov/pubmed/26636946
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.10.035
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