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De novo clustering methods outperform reference-based methods for assigning 16S rRNA gene sequences to operational taxonomic units

Background. 16S rRNA gene sequences are routinely assigned to operational taxonomic units (OTUs) that are then used to analyze complex microbial communities. A number of methods have been employed to carry out the assignment of 16S rRNA gene sequences to OTUs leading to confusion over which method i...

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Bibliographische Detailangaben
Veröffentlicht in:PeerJ
Hauptverfasser: Westcott, Sarah L., Schloss, Patrick D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: PeerJ Inc. 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4675110/
https://ncbi.nlm.nih.gov/pubmed/26664811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.1487
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