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A method for sorting and pairwise selection of nanobodies for the development of highly sensitive sandwich immunoassays

Single domain heavy-chain binders (nanobodies) obtained from camelid antibody libraries hold a great promise for immunoassay development. However, there is no simple method to select the most valuable nanobodies from the crowd of positive clones obtained after the initial screening. In this paper, w...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Rossotti, Martín A., Pirez, Macarena, Gonzalez-Techera, Andres, Cui, Yongliang, Bever, Candace S., Lee, Kin S. S., Morisseau, Christophe, Leizagoyen, Carmen, Gee, Shirley, Hammock, Bruce D., González-Sapienza, Gualberto
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666776/
https://ncbi.nlm.nih.gov/pubmed/26544909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.5b03561
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