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β-Hydroxysterol distribution as determined by freeze-fracture cytochemistry
Filipin, a polyene antibiotic, fluoresces and forms 15–25 nm aggregates when combined with β-hydroxysterols, rendering sterols detectable by fluorescence microscopy and by electron microscopy of thin sections and freeze-fracture replicas. We applied filipin in a glutaraldehyde fixative to tissue-cul...
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| Publicado no: | Histochem J |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4666710/ https://ncbi.nlm.nih.gov/pubmed/6796549 |
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