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Improvements to a Markerless Allelic Exchange System for Bacillus anthracis

A system was previously developed for conducting I-SceI-mediated allelic exchange in Bacillus anthracis. In this system, recombinational loss of a chromosomally-integrated allelic exchange vector is stimulated by creation of a double-stranded break within the vector by the homing endonuclease I-SceI...

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Publicat a:PLoS One
Autors principals: Plaut, Roger D., Stibitz, Scott
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666636/
https://ncbi.nlm.nih.gov/pubmed/26624016
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0142758
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