Blocking of targeted microRNAs from next-generation sequencing libraries

Highly abundant microRNAs (miRNAs) in small RNA sequencing libraries make it difficult to obtain efficient measurements of more lowly expressed species. We present a new method that allows for the selective blocking of specific, abundant miRNAs during preparation of sequencing libraries. This techni...

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Библиографические подробности
Опубликовано в: :Nucleic Acids Res
Главные авторы: Roberts, Brian S., Hardigan, Andrew A., Kirby, Marie K., Fitz-Gerald, Meredith B., Wilcox, C. Mel, Kimberly, Robert P., Myers, Richard M.
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2015
Предметы:
Methods Online
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4666382/
https://ncbi.nlm.nih.gov/pubmed/26209131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv724
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