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Development and validation of a sample sparing strategy for HLA typing utilizing next generation sequencing

We report the development of a general methodology to genotype HLA class I and class II loci. A Whole Genome Amplification (WGA) step was used as a sample sparing methodology. HLA typing data could be obtained with as few as 300 cells, underlining the usefulness of the methodology for studies for wh...

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Detalhes bibliográficos
Publicado no:Hum Immunol
Main Authors: McKinney, Denise M., Fu, Zheng, Le, Lucas, Greenbaum, Jason A., Peters, Bjoern, Sette, Alessandro
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4662932/
https://ncbi.nlm.nih.gov/pubmed/26027778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.humimm.2015.04.007
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