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Slow Off-rates and Strong Product Binding Are Required for Processivity and Efficient Degradation of Recalcitrant Chitin by Family 18 Chitinases

Processive glycoside hydrolases are the key components of enzymatic machineries that decompose recalcitrant polysaccharides, such as chitin and cellulose. The intrinsic processivity (P(Intr)) of cellulases has been shown to be governed by the rate constant of dissociation from polymer chain (k(off))...

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Bibliographische Detailangaben
Veröffentlicht in:J Biol Chem
Hauptverfasser: Kurašin, Mihhail, Kuusk, Silja, Kuusk, Piret, Sørlie, Morten, Väljamäe, Priit
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4661419/
https://ncbi.nlm.nih.gov/pubmed/26468285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.684977
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