The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo

INTRODUCTION: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has been used to manipulate gene expression in vitro. NEW...

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Detalles Bibliográficos
Publicado en:J Neurosci Methods
Main Authors: Lewis, Jo E., Brameld, John M., Hill, Phil, Barrett, Perry, Ebling, Francis J.P., Jethwa, Preeti H.
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier/North-Holland Biomedical Press 2015
Assuntos:
Basic Neuroscience
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4659456/
https://ncbi.nlm.nih.gov/pubmed/26300182
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2015.08.013
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