Deletion of Helicobacter pylori vacuolating cytotoxin gene by introduction of directed mutagenesis

AIM: To construct a vacA-knockout Helicobacter pylori mutant strain, whose only difference from the wild strain is its disrupted vacA gene. METHODS AND RESULTS: A clone containing kanamycin resistance gene used for homologous recombination was constructed in a directional cloning procedure into pBlu...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:World J Gastroenterol
Main Authors: Yuan, Jian-Ping, Li, Tao, Shi, Xiao-Dong, Hu, Bao-Yu, Yang, Gui-Zhen, Tong, Shan-Qing, Guo, Xiao-Kui
Format: Artigo
Jezik:Inglês
Izdano: Baishideng Publishing Group Inc 2003
Teme:
H Pylori
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4656472/
https://ncbi.nlm.nih.gov/pubmed/14562387
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i10.2251
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