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Proteome-wide enrichment of proteins modified by lysine methylation
We present a protocol for using the triple malignant brain tumor domains of L3MBTL1 (3×MBT), which bind to mono- and di-methylated lysine with minimal sequence specificity, in order to enrich for such methylated lysine from cell lysates. Cells in culture are grown with amino acids containing light o...
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| Udgivet i: | Nat Protoc |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4651005/ https://ncbi.nlm.nih.gov/pubmed/24309976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.164 |
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