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Proteome-wide enrichment of proteins modified by lysine methylation

We present a protocol for using the triple malignant brain tumor domains of L3MBTL1 (3×MBT), which bind to mono- and di-methylated lysine with minimal sequence specificity, in order to enrich for such methylated lysine from cell lysates. Cells in culture are grown with amino acids containing light o...

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Bibliografiska uppgifter
I publikationen:Nat Protoc
Huvudupphovsmän: Carlson, Scott M, Moore, Kaitlyn E, Green, Erin M, Martín, Glòria Mas, Gozani, Or
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2013
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4651005/
https://ncbi.nlm.nih.gov/pubmed/24309976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.164
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