Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing

Protein trans-splicing using split inteins is well established as a useful tool for protein engineering. Here we show, for the first time, that this method can be applied to a membrane protein under native conditions. We provide compelling evidence that the heptahelical proteorhodopsin can be assemb...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Mehler, Michaela, Eckert, Carl Elias, Busche, Alena, Kulhei, Jennifer, Michaelis, Jonas, Becker-Baldus, Johanna, Wachtveitl, Josef, Dötsch, Volker, Glaubitz, Clemens
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2015
Assuntos:
Molecular Biophysics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4646914/
https://ncbi.nlm.nih.gov/pubmed/26405032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.681205
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados