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Dependence of Two-Photon eGFP Bleaching on Femtosecond Pulse Spectral Amplitude and Phase
Photobleaching is a key limitation in two-photon imaging of fluorescent proteins with femtosecond pulsed excitation. We present measurements of the dependence of eGFP photobleaching on the spectral amplitude and phase of the pulses used. A strong dependence on the excitation wavelength was confirmed...
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| Publicado no: | J Fluoresc |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4644685/ https://ncbi.nlm.nih.gov/pubmed/26411799 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10895-015-1667-1 |
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