Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene amplification

To date, characterization of ancient oral (dental calculus) and gut (coprolite) microbiota has been primarily accomplished through a metataxonomic approach involving targeted amplification of one or more variable regions in the 16S rRNA gene. Specifically, the V3 region (E. coli 341–534) of this gen...

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Udgivet i:Sci Rep
Main Authors: Ziesemer, Kirsten A., Mann, Allison E., Sankaranarayanan, Krithivasan, Schroeder, Hannes, Ozga, Andrew T., Brandt, Bernd W., Zaura, Egija, Waters-Rist, Andrea, Hoogland, Menno, Salazar-García, Domingo C., Aldenderfer, Mark, Speller, Camilla, Hendy, Jessica, Weston, Darlene A., MacDonald, Sandy J., Thomas, Gavin H., Collins, Matthew J., Lewis, Cecil M., Hofman, Corinne, Warinner, Christina
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4643231/
https://ncbi.nlm.nih.gov/pubmed/26563586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep16498
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