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Reconstituting Intracellular Vesicle Fusion Reactions: The Essential Role of Macromolecular Crowding
Intracellular vesicle fusion is mediated by SNAREs and Sec1/Munc18 (SM) proteins. Despite intensive efforts, the SNARE-SM mediated vesicle fusion reaction has not been faithfully reconstituted in biochemical assays. Here, we present an unexpected discovery that macromolecular crowding is required fo...
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| Publicado no: | J Am Chem Soc |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4638180/ https://ncbi.nlm.nih.gov/pubmed/26431309 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.5b08306 |
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