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mRNA analysis of intracytoplasmically-stained, FACS-purified pancreatic islet cells using the quantitative nuclease protection assay
Exploring the pathophysiology underlying diabetes mellitus requires characterizing the cellular constituents of pancreatic islets, primarily insulin-producing β-cells. Such efforts have been limited by inadequate techniques for purifying islet cellular subsets for further biochemical and gene-expres...
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| Publicado no: | Nat Biotechnol |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4638177/ https://ncbi.nlm.nih.gov/pubmed/19838197 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1579 |
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