Synthesis of a gene for sensory rhodopsin I and its functional expression in Halobacterium halobium.

We have designed, synthesized, and expressed in Halobacterium halobium a gene encoding sensory rhodopsin I (SR-I). The gene has been optimized for cassette mutagenesis by incorporating 30 unique restriction sites with uniform spacing throughout the 720-bp coding region. For expression, the coding re...

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Main Authors: Krebs, M P, Spudich, E N, Khorana, H G, Spudich, J L
Format: Artigo
Jezik:Inglês
Izdano: 1993
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC46325/
https://ncbi.nlm.nih.gov/pubmed/8475097
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