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Evaluation of Colorimetric Assays for Analyzing Reductively Methylated Proteins: Biases and Mechanistic Insights
Colorimetric protein assays, such as the Coomassie blue G-250 dye-binding (Bradford) and bicinchoninic acid (BCA) assays, are commonly used to quantify protein concentration. The accuracy of these assays depends on the amino acid composition. Because of the extensive use of reductive methylation in...
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Publicado no: | Anal Biochem |
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Main Authors: | , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4631703/ https://ncbi.nlm.nih.gov/pubmed/26342307 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.08.027 |
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