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Evaluation of Colorimetric Assays for Analyzing Reductively Methylated Proteins: Biases and Mechanistic Insights

Colorimetric protein assays, such as the Coomassie blue G-250 dye-binding (Bradford) and bicinchoninic acid (BCA) assays, are commonly used to quantify protein concentration. The accuracy of these assays depends on the amino acid composition. Because of the extensive use of reductive methylation in...

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Detalhes bibliográficos
Publicado no:Anal Biochem
Main Authors: Brady, Pamlea N., Macnaughtan, Megan A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4631703/
https://ncbi.nlm.nih.gov/pubmed/26342307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.08.027
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